England manager Gareth Southgate named four right-backs in the final of his 26-man European squad on Tuesday after he ousted six players, including fit midfielder Jesse Lingard.
Defenders Ben Godfrey and Ben White, goalkeeper Aaron Ramsdale, striker Ollie Watkins, and midfielder James Ward-Prowse were among others who failed to make the cut.
Manchester United striker Mason Greenwood was previously withdrawn with an injury that reduced the team to a provisional 33 called up a week earlier.
Liverpool’s Alexander-Arnold was one of four defenders on the right. Injured duo Harry Maguire and Jordan Henderson were both involved.
The FA said the resigned players would remain in the group for two friendly matches ahead of the Middlesbrough tournament against Austria on Wednesday and Romania on Sunday.
Teams competing in Euro 2020 can make unlimited substitutions before the first match due to serious injury or illness.
Fifteen out of 26 seniors will get their first experience in international tournaments. Starting from June 11 till July 11 Euro 2020 takes place across Europe, with the semi-finals and final at Wembley Stadium in London, as well as several group stage games.
England’s Euro 2020 Group D campaign begins with games against Croatia, Scotland and the Czech Republic.
Goalkeepers: Dean Henderson (Manchester United), Sam Johnstone (West Bromwich Albion), Jordan Pickford (Everton)
Defenders: Trent Alexander-Arnold (Liverpool), Ben Chilwell (Chelsea), Conor Coady (Wolverhampton Wanderers), Reece James (Chelsea), Harry Maguire (Manchester United), Tyrone Mings (Aston Villa), Luke Shaw (Manchester United), John Stones (Manchester City), Kieran Trippier (Atletico Madrid), Kyle Walker (Manchester City)
Midfielders: Jude Bellingham (Borussia Dortmund), Jordan Henderson (Liverpool), Mason Mount (Chelsea), Kalvin Phillips (Leeds United), Declan Rice (West Ham United)
Forward: Dominic Calvert-Lewin (Everton), Phil Foden (Manchester City), Jack Grealish (Aston Villa), Harry Kane (Tottenham Hotspur), Marcus Rashford (Manchester United), Bukayo Saka (Arsenal), Jadon Sancho (Borussia Dortmund) ), Raheem Sterling (Manchester City)